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+BE AWARE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also among the amino groups within the spot areas do not form bonds with the amino acids. BE AWARE: This avoids the coupling of amino acids within the additional cycles with the membrane as an alternative of the growing peptide chain. Be certain all the purification buffers are at four °C and perform the protein purification within the chilly room to avoid protein denaturation.
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+Widespread posttranslational modifications include methylation of the ε-amino group, giving methyl- , dimethyl- , and trimethyllysine The latter occurs in calmodulin Other posttranslational modifications at lysine residues embrace acetylation , sumoylation , and ubiquitination Collagen contains hydroxylysine , which is derived from lysine by lysyl hydroxylase - Glycosylation of hydroxylysine residues within the endoplasmic reticulum or Golgi equipment is used to mark sure proteins for secretion from the cell In opsins like rhodopsin and the visible opsins (encoded by the genes OPN1SW , OPN1MW , and OPN1LW ), retinaldehyde kinds a Schiff base with a conserved lysine residue, and interplay of light with the retinylidene group causes sign transduction in color vision (See visible cycle for details).[yahoo.com](https://www.yahoo.com/)